Improved Synthesis of Oligodeoxyribonucleotides by Solid‐Phase Phosphotriester Method Utilizing O6‐[2‐(p‐Nitrophenyl)ethyl]‐2′‐deoxyguanosine Derivatives
- 8 August 1984
- journal article
- research article
- Published by Wiley in Helvetica Chimica Acta
- Vol. 67 (5) , 1356-1364
- https://doi.org/10.1002/hlca.19840670521
Abstract
The synthesis of oligodeoxyribonucleotides on a cross‐linked polystyrene solid support utilizing stable mono‐ and dinucleotide phosphotriester building blocks is presented. The use of O6[2‐(p‐nitrophenyl)ethyl]‐2′‐deoxyguanosine derivatives yields cleaner DNA fragments by suppressing side reactions. Modifications improving the phosphotriester methodology are presented. The purification methods and analysis of synthetic oligodeoxyribonucleotides are described.This publication has 37 references indexed in Scilit:
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