Kinetic Characterization of the Glycosyltransferase Module of Staphylococcus aureus PBP2
Open Access
- 15 March 2005
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 187 (6) , 2215-2217
- https://doi.org/10.1128/jb.187.6.2215-2217.2005
Abstract
We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.Keywords
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