Sequence Diversity of Nuclear and Polysomal Polyadenylated and Non‐polyadenylated RNA in Normal and Regenerating Rat Liver

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Abstract
A DNA probe purified from RNA .cntdot. DNA hybrids of total sham-operated liver RNA and non-repetitive DNA was used to show that nuclear poly(A)-rich RNA from sham-operated liver and from 2.5 h and 48 h regenerating liver contains .apprx. 50% of the complexity of total liver RNA. The differences between normal and regenerating liver occur in the poly(A)-free fraction of nuclear RNA. At the polysome level, it was found that polysomal RNA has 1/3 of the sequence diversity of total RNA, and of this, approximately 65% can be accounted for by poly(A)-rich and 55% by poly(A)-free molecules. When DNA probes were prepared from hybrids formed using polysomal RNA from sham-operated liver and regenerating liver at 2.5 h and 48 h post-hepatectomy and then employed in reactions with homologous and heterologous RNA, no differences were detectable between either normal and regenerating liver or regenerating liver at times during hypertrophy and hyperplasia.