Study of the feasibility of immunochemical methods for identification of pleuronectid eggs

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Abstract
Fertilized eggs of seven species of the family Pleuronectidae were classified into two groups based on the presence or absence of an antigen (A) detected by both immunodiffusion and immunofluorescence. The immune sera used were produced by rabbits in response to intraperitoneal injections of homogenates of whole egg extracts and Freund's Complete Adjuvant. All species tested possessed a common antigen (B) whereas only four of the seven species possessed an additional antigen (A). The antisera produced with fresh and frozen eggs did not detectably react with chemically preserved eggs in either immunofluorescent or immunodiffusion test. Temperature of incubation was an important factor for the immunofluorescent method; fish eggs incubated at 37°C fluoresced with variable intensities, while at 0°C all eggs fluoresced with equal intensity.

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