Sensitive Chemiluminescence Enzyme Immunoassay for Vascular Endothelial Growth Factor/Vascular Permeability Factor in Human Serum

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Abstract
A sandwich chemiluminescence enzyme immunoassay for measuring the level of VEGF/VPF in serum was constructed. The detectability of the assay is very low (1.0 pg/ml) and the measurable range of the assay was very wide (1-1000 pg/ml). The assay showed that the average level of VEGF/VPF in human sera from healthy blood donors was approximately 19 pg/ml.