To Investigate whether T cells recognizing the same HLA molecule may demonstrate homology in parts of their TCR, five different HLA-DQw8-specific T lymphocyte clones (TLC) were studied. The TCRα and -β genes of four alloreactive, HLA-DQw8-spedfic and one antigen-specific TLC were sequenced. All TLC used different Vα and Vβ genes. However, four of the TLC shared a certain CDR1β motif and all five used either Jβ2.3 or -2.5. In addition, two used the same Jα. The results Indicate a possible preferential usage of certain TCR structures by T cells specific for DQw8.