In Vitro Transcription by RNA Polymerase Associated with Rice Gall Dwarf Virus
- 1 March 1984
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 65 (3) , 533-538
- https://doi.org/10.1099/0022-1317-65-3-533
Abstract
Summary An RNA-dependent polymerase associated with rice gall dwarf virus (RGDV), a new Phytoreovirus, was characterized. Optimum polymerase activity was found between pH 8.0 and 8.5 at 25°C. Mg2+ was essential for the activity, the optimum concentration being 2 mm. Mn2+ could replace Mg2+. The RNA products synthesized in vitro hybridized with RGDV RNA, but not with RNA of rice dwarf virus which is in the same subgroup of plant reoviruses. Electrophoretic analysis of the products showed at least nine bands, the sizes of which are discussed.Keywords
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