Bacterial chromosome extraction and isolation
- 7 November 2002
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 2 (4) , 207-212
- https://doi.org/10.1039/b208010a
Abstract
We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.Keywords
This publication has 8 references indexed in Scilit:
- Electrostatic manipulation of DNA in microfabricated structuresPublished by Institute of Electrical and Electronics Engineers (IEEE) ,2003
- Electrodeless Dielectrophoresis of Single- and Double-Stranded DNABiophysical Journal, 2002
- Hydrodynamic Focusing on a Silicon Chip: Mixing Nanoliters in MicrosecondsPhysical Review Letters, 1998
- The Complete Genome Sequence of Escherichia coli K-12Science, 1997
- Biotechnology at low Reynolds numbersBiophysical Journal, 1996
- Molecular dielectrophoresis of biopolymersIEEE Transactions on Industry Applications, 1994
- Novel method of cell fusion in field constriction area in fluid integration circuitIEEE Transactions on Industry Applications, 1989
- Conformational transitions in the Escherichia coli chromosome: Analysis by viscometry and sedimentationJournal of Molecular Biology, 1975