Correlation between neutrophil superoxide formation, luminol-augmented chemiluminescence and intracellular Ca2+levels upon stimulation with leukotriene B4, formylpeptide and phorbolester

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Abstract
When neutrophils were stimulated with leukotriene B4 (LTB4) O-production and elevation of intracellular Ca2+ were initiated more rapidly than after stimulation with, N-formyl-methionyl-leucyl-phenylalanine. Phorbol myristate acetate (PMA) induced a substantially more protracted superoxide (O2) production. Luminol augmented chemiluminescence (LCL) reflected the kinetic characteristics accurately. The primary distinguishing attribute of LTB4-induced responses were an initial high rate of activation and a rapid termination. Thus, LTB4-induced neutrophil Ca2+-inflow and O2-formation may be initiated by mechanisms similar in part to those for formylpeptides; however it does not possess the ability to maintain this activation. The kinetic properties of neutrophil activation may be studied with LCL, irrespective of the exact nature of the LCL-generating oxidants formed by neutrophils.

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