Elisa for Herpes Simplex Virus (HSV) Type‐Specific Antibodies in Human Sera Using HSV Type 1 and Type 2 Polyspecific Antigens Blocked with Type‐Heterologous Rabbit Antibodies

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Abstract
Crude antigenic preparations made from rabbit cornea cells infected with either HSV type 1 or type 2 could be used in ELISA for titration of HSV type‐specific antibodies in human sera. After immunochemical binding of the crude HSV antigens in microtitre wells by use of type‐homologous rabbit antibodies, type‐common antigenic sites were blocked with type‐heterologous rabbit antibodies. Titration of human sera in this system showed that high concentrations of type‐heterologous rabbit antibodies were capable of completely blocking type‐common antigenic sites, while leaving type‐specific antigenic sites unblocked and capable of reacting with human antibodies. Thus HSV type‐specific antibodies in human sera could be measured in ELISA without the use of purified typespecific antigens.