Effects of Cultivation Temperature on Fatty Acid Composition in Rhodotorula gracilis.

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Abstract
The lipid content of R. gracilis decreases when the cultivation temperature is increased from 27 to 35*C. In parallel cultures run at the same temperature, increased lipid content implies an increased percentage of fatty-acids in the lipid fraction. Fatty-acid composition varies with cultivation temperature. Oleic acid increases, linoleic acid decreases, and linolenic acid increases strikingly with decreasing temperature, while the saturated fatty-acids remain unchanged. The temperature during the first cultivation phase in which normal cells are produced, has some influence on the fatty-acid composition. This influence persists during the second growth phase in which lipid is accumulated, but the temperature during this phase has a much more distinct effect on the formation of unsaturated acids, especially linolenic acid.

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