Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes
- 1 March 1989
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 81 (4) , 358-362
- https://doi.org/10.1007/bf00283691
Abstract
A technique of in situ hybridization on metaphase chromosomes with biotinylated DNA probes is described. This technique was used to localize unique DNA sequences on chromosomes and allowed a localization of two probes 1.8 and 1.3 kb long. The hybridization signal appears like two, twin, spots on the two sister chromatids, allowing a clear distinction from the background. Moreover a chromosomal localization is possible by counting a relatively small number of mitoses compared with the technique using 3H-labeled DNA probes.This publication has 25 references indexed in Scilit:
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