Measurement of Cytolytic Antibody in Experimental Chagas' Disease Using a Terminal Radiolabeling Procedure

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Abstract
The onset and development of anti-Trypanosoma cruzi cytolytic antibody during the course of infection were examined in 2 strains of mice that differ in susceptibility to T. cruzi. The relative amounts of cytolytic antibody in the sera of mice were determined by a terminal radiolabeling procedure that measures the amount of 3H-thymidine incorporated into culture-form parasites that survive treatment with antibody and complement. Low levels of anti-T. cruzi cytolytic antibody developed during the course of infection in mice; there was no apparent difference in cytolytic antibody responses during the acute phase of infection in relatively resistant C57BL/6 mice and highly susceptible C3H(He) mice.