Autoradiographic Studies on the Kinetics of Nuclear-Cytoplasmic Transfer of RNA in Blast Cells of Acute Leukaemia

Abstract
Autoradiographic investigations on normal PHA- timulated lymphocytes and on blast cells of acute leukaemia were carried out in order to evaluate the nuclear-cytoplasmic shift of 3Huridine and the degree of nucleolar labelling with the same radioactive precursor. The results obtained indicate that, in contrast with PHA blast cells, a variable proportion of leukaemic blasts, even after prolonged periods of incubation, show a delayed transfer of uridine to the cytoplasm, as well as a reduced concentration of label in the nucleoli. The implications of these findings are discussed, also in the light of recent biochemical acquisitions

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