A technique for electron microscope autoradiography (and X‐ray microanalysis) of diffusible substances using freeze‐dried fresh frozen sections

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Abstract
SUMMARY: Several attempts have been made over the past few years to localize diffusible radioactive substances in tissues at the electron microscope level. The procedures employed hitherto have involved one or more aspects of the methods used in tissue preparation for conventional electron microscopy and as such are unlikely to be universally applicable for all classes of diffusible substance. The present paper shows that the use of ultrathin frozen sections of fresh unembedded biological material permits the ultrastructural localization by autoradiography and X‐ray microanalysis of highly soluble electrolytes.