ORIGINAL H‐2d AND FOREIGN H ‐ 2k ‐ LIKE ANTIGENS ARE INDEPENDENT ENTITIES ON A CHEMICALLY INDUCED SARCOMA

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Abstract
SUMMARY: We have previously shown that a methylcholanthrene‐induced sarcoma of BALB/c strain (C‐1) expressed, in addition to its original H‐2d antigens, foreign H‐2k‐like determinants. In the present study the relationship between H‐2d and H‐2k‐like antigens was examined by in vitro and in vivo assays. Cultured tumour cells were exposed in the cold to either a monospecific (D‐23, D‐25, D‐1) or polyspecific (BALB/c anti‐C3Hf) alloantiserum directed to H‐2k specificities and then used to absorb the cytotoxic activity of either monospecific (D‐31) or a polyspecific (C3Hf anti‐BALB/c) alloantisera to H‐2d antigens (blocking test). The opposite was also done, i.e. tumour cells were coated with anti‐H‐2d sera and used to absorb the cytotoxicity of anti‐H‐2k sera. No reciprocal interference was found between the two H‐2‐different antigens in the absorption of related alloantisera. Suspensions of irradiated C‐1 tumour cells were exposed in vitro to either anti‐H‐2d or anti‐H‐2k antisera and then used to immunize either syngeneic BALB/c or allogeneic C3Hf mice. The coating of immunizing neoplastic cells with BALB/c anti‐C3Hf serum prevented anti‐C3Hf (anti‐H‐2k) antibody production in BALB/c mice without affecting anti‐BALB/c (anti‐H‐2d) antibody development in C3Hf animals; coating of H‐2d antigens on tumour cells strongly reduced anti‐BALB/c but not anti‐C3Hf antibody production. Both in vitro and in vivo experiments indicated that foreign H‐2k‐like determinants are physically separated from the wild H‐2d antigens on the C‐1 sarcoma cells.