ETUDE DE L'ACETAMIDASE D'UNE SOUCHE DE BREVIBACTERIUM

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Abstract
The acetamidase of Brevibacterium R312, a strain with nitrilase activity, has very different characteristics from those of its acetonitrilase. This acetamidase presents an activity for pH values greater than 3 and has an optimum activity at pH 7. The optimal temperature is between 60.degree.-70.degree. C. The enzyme resists high temperatures and its Km is 3.5 .times. 10-3 M. The -SH, -OH, -NH2, .**GRAPHIC**. and tryptophan groups seem to be necessary for the acetamidase activity. The enzyme remains in supernatant fluid from the centrifugation at 180,000 .times. g. It is and adpative enzyme. This acetamidase looks like aliphatic amidases described by others.