Human dendritic cell subsets in NOD/SCID mice engrafted with CD34+ hematopoietic progenitors
- 1 November 2003
- journal article
- Published by American Society of Hematology in Blood
- Vol. 102 (9) , 3302-3310
- https://doi.org/10.1182/blood-2003-02-0384
Abstract
Distinct human dendritic cell (DC) subsets differentially control immunity. Thus, insights into their in vivo functions are important to understand the launching and modulation of immune responses. We show that nonobese diabetic/LtSz-scid/scid (NOD/SCID) mice engrafted with human CD34+ hematopoietic progenitors develop human myeloid and plasmacytoid DCs. The skin displays immature DCs expressing Langerin, while other tissues display interstitial DCs. Myeloid DCs from these mice induce proliferation of allogeneic CD4 T cells in vitro, and bone marrow human cells containing plasmacytoid DCs release interferon-α (IFN-α) upon influenza virus exposure. Injection of influenza virus into reconstituted mice triggers IFN-α release and maturation of mDCs. Thus, these mice may provide a model to study the pathophysiology of human DC subsets.Keywords
This publication has 50 references indexed in Scilit:
- Human epithelial cells trigger dendritic cell–mediated allergic inflammation by producing TSLPNature Immunology, 2002
- Generation of Human CD8 T Regulatory Cells by CD40 Ligand–activated Plasmacytoid Dendritic CellsThe Journal of Experimental Medicine, 2002
- Long-Term Engraftment of Nonobese Diabetic/Severe Combined Immunodeficient Mice with Human CD34+Cells Transduced by a Self-Inactivating Human Immunodeficiency Virus Type 1 VectorHuman Gene Therapy, 2001
- Arrest of human dendritic cells at the CD34−/CD4+/HLA-DR+ stage in the bone marrow of NOD/SCID-human chimeric miceBlood, 2001
- The Induction of Tolerance by Dendritic Cells That Have Captured Apoptotic CellsThe Journal of Experimental Medicine, 2000
- THE CD1 SYSTEM: Antigen-Presenting Molecules for T Cell Recognition of Lipids and GlycolipidsAnnual Review of Immunology, 1999
- Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha.The Journal of Experimental Medicine, 1994
- Thymic selection of the human T cell receptor V beta repertoire in SCID-hu mice.The Journal of Experimental Medicine, 1992
- Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor.The Journal of Experimental Medicine, 1992
- GM-CSF and TNF-α cooperate in the generation of dendritic Langerhans cellsNature, 1992