Flow Cytometric Measurement of Rna Synthesis Based on Bromouridine Labelling and Combined with Measurement of Dna Content or Cell Surface Antigen
Open Access
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis in Acta Oncologica
- Vol. 32 (5) , 521-524
- https://doi.org/10.3109/02841869309096111
Abstract
RNA synthesis can be analysed in nuclei or cells labelled with 5-bromouridine (BrUrd) and stained using cross-reacting anti-bromodeoxyuridine (BrdUrd) antibody. Flow cytometric dual parameter analysis of BrUrd incorporation and DNA content in nuclear suspensions of human blood lymphocytes showed that RNA synthesis increased within the first 24 hours of phytohemagglutinin (PHA) stimulation, reaching a maximum at 48 hours, when cells had entered the cell cycle. Using a new method for flow cytometric dual parameter analysis of BrUrd incorporation and a cell surface antigen, spontaneous RNA synthesis in HL-60 and K-562 cells was measured simultaneous with CD13 expression.Keywords
This publication has 3 references indexed in Scilit:
- Flow cytometric measurement bof RNA synthesis using bromouridine labelling and bromodeoxyuridine antibodiesCytometry, 1993
- Simultaneous identification of eight leucocyte subsets of human peripheral blood using three-colour immunofluorescence flow cytometric analysisJournal of Immunological Methods, 1991
- G 1 Events and Regulation of Cell ProliferationScience, 1989