EFFECTS OF FUSOBACTERIUM-NECROPHORUM LEUKOTOXIN ON RABBIT PERITONEAL MACROPHAGES INVITRO

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 38  (4) , 491-495
Abstract
A method to demonstrate leukotoxic activity of F. necrophorum in vitro is described. A continuous dialysis sac culture system, using reduced liquid medium was used to grow F. necrophorum for 7 day periods. The continuous culture dialysis filtrate contained leukotoxic substance(s) which appeared to be less than 10,000 in MW, heat resistant and stable at 4.degree. C for at least 10 days. Leukotoxic activity was demonstrated in vitro by determining the percentage of macrophages taking up trypan blue dye after these were exposed to a 1:2 solution of continuous culture dialysis filtrate and Eagle''s minimal essential medium at 1, 2, 4 and 6 h. Approximately 90% of these macrophages were destroyed during the 6 h incubation period.

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