Construction and Synchronization of dnaA Temperature-Sensitive Mutants of Streptomyces
- 15 February 2002
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (4) , 1214-8
- https://doi.org/10.1128/jb.184.4.1214-1218.2002
Abstract
Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39°C replication in the mutants ceased in about 90 min but resumed on return to 30°C. This allowed manipulations to achieve replication synchronization.Keywords
This publication has 15 references indexed in Scilit:
- Interactions of the Streptomyces lividans initiator protein DnaA with its targetEuropean Journal of Biochemistry, 1999
- A 'Gram-negative-type' DNA polymerase III is essential for replication of the linear chromosome of Streptomyces coelicolor A3(2)Molecular Microbiology, 1999
- Structural elements of the Streptomyces oriC region and their interactions with the DnaA proteinMicrobiology, 1998
- The chromosomal DNA of Streptomyces lividans 66 is linearMolecular Microbiology, 1993
- Isolation and characterization of temperature-sensitive mutants of Streptomyces coelicolor A3(2) blocked in macromolecular synthesisJournal of General Microbiology, 1992
- Structure and function of DnaA and the DnaA‐box in eubacteria: evolutionary relationships of bacterial replication originsMolecular Microbiology, 1991
- The pMTL nic− cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencingGene, 1988
- Genetic instability and DNA amplification in Streptomyces lividans 66Journal of Bacteriology, 1987
- Allele-specific suppression of dnaA (Ts) mutations by rpoB mutations in Escherichia coliMolecular Genetics and Genomics, 1984
- Plasmids, Recombination and Chromosome Mapping in Streptomyces lividans 66Microbiology, 1983