Nucleolar Definition in Hemalum and Eosin Staining

1 January 1962

journal article
research article
Published by Taylor & Francis in Stain Technology

Vol. 37 (1) , 41-42
https://doi.org/10.3109/10520296209114568

Abstract

This investigation was designed to clarify why routine hemalum and eosin staining so frequently demonstrates nucleoli in a distinctive purple to red shade. Participation of eosin in the effect suggests that nucleolar basic protein is being stained, and this fact has been confirmed with bromphenol blue. Dye affinities of the nucleolus have been further studied by successively substituting basic and acid fuchsin for the pyronin in the standard Unna-Pappenheim mixture. At pH 4.8 it appears that the nucleolus has both acid and basic groups freely available for staining.

Keywords

This publication has 2 references indexed in Scilit:

THE CYTOCHEMICAL STAINING AND MEASUREMENT OF PROTEIN WITH MERCURIC BROMPHENOL BLUE
The Biological Bulletin, 1953
The quantitative estimation of desoxyribosenucleic acid based on methyl green staining
Experimental Cell Research, 1950