Cardiac Ankyrin Repeat Protein Is a Novel Marker of Cardiac Hypertrophy
- 1 July 2000
- journal article
- other
- Published by Wolters Kluwer Health in Hypertension
- Vol. 36 (1) , 48-53
- https://doi.org/10.1161/01.hyp.36.1.48
Abstract
—CARP, a cardiac doxorubicin (adriamycin)-responsive protein, has been identified as a nuclear protein whose expression is downregulated in response to doxorubicin. In the present study, we tested the hypothesis that CARP serves as a reliable genetic marker of cardiac hypertrophy in vivo and in vitro. CARP expression was markedly increased in 3 distinct models of cardiac hypertrophy in rats: constriction of abdominal aorta, spontaneously hypertensive rats, and Dahl salt-sensitive rats. In addition, we found that CARP mRNA levels correlate very strongly with the brain natriuretic peptide mRNA levels in Dahl rats. Transient transfection assays into primary cultures of neonatal rat cardiac myocytes indicate that transcription from the CARP and brain natriuretic peptide promoters is stimulated by overexpression of p38 and Rac1, components of the stress-activated mitogen-activated protein kinase pathways. Mutation analysis and electrophoretic mobility shift assays indicated that the M-CAT element can s...Keywords
This publication has 32 references indexed in Scilit:
- Tumor Necrosis Factor-α Decreases the Phosphorylation Levels of Phospholamban and Troponin I in Spontaneously Beating Rat Neonatal Cardiac MyocytesJournal of Molecular and Cellular Cardiology, 1999
- A requirement for the rac1 GTPase in the signal transduction pathway leading to cardiac myocyte hypertrophy.Journal of Clinical Investigation, 1998
- Stimulation of the p38 Mitogen-activated Protein Kinase Pathway in Neonatal Rat Ventricular Myocytes by the G Protein–coupled Receptor Agonists, Endothelin-1 and Phenylephrine: A Role in Cardiac Myocyte Hypertrophy?The Journal of cell biology, 1998
- Differential Effects of Protein Kinase C, Ras, and Raf-1 Kinase on the Induction of the Cardiac B-type Natriuretic Peptide Gene through a Critical Promoter-proximal M-CAT ElementJournal of Biological Chemistry, 1997
- The Role of Transcription Enhancer Factor-1 (TEF-1) Related Proteins in the Formation of M-CAT Binding Complexes in Muscle and Non-muscle TissuesPublished by Elsevier ,1996
- Integration of MAP Kinase Signal Transduction Pathways at the Serum Response ElementScience, 1995
- Selective activation of the JNK signaling cascadeand c-Jun transcriptional activity by the small GTPases Rac and Cdc42HsCell, 1995
- A MAP Kinase Targeted by Endotoxin and Hyperosmolarity in Mammalian CellsScience, 1994
- Transcriptional Regulation During Cardiac Growth and DevelopmentAnnual Review of Physiology, 1993
- Role of Genetic Factors in Susceptibility to Experimental Hypertension due to Chronic Excess Salt IngestionNature, 1962