Fluorometric Assay of Diphenylhydantoin in Plasma or Whole Blood
- 1 July 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 18 (7) , 675-676
- https://doi.org/10.1093/clinchem/18.7.675
Abstract
A highly sensitive assay procedure for 5,5-diphenylhydantoin (DPH) involves oxidation with alkaline permanganate to form benzophenone. This is then extracted with heptane, allowed to react with concentrated sulfuric acid, and the fluorescence of the reaction product is measured. The procedure appears to be fairly specific for DPH; 5-(p-hydroxyphenyl)-5-phenylhydantoin (a DPH metabolite), phenobarbital, or other anticonvulsant drugs do not interfere. The sensitivity of the fluorometric procedure permits assays on finger-blood specimens. It provides a simple means for evaluating the effectiveness of a given dose schedule in different subjects.Keywords
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