Separation and Partial Purification of Plasma-Membrane Fragments from Ehrlich Ascites Carcinoma Microsomes
- 4 June 1965
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 148 (3675) , 1343-1345
- https://doi.org/10.1126/science.148.3675.1343
Abstract
Membrane vesicles arising from the surface of Ehrlich ascites carcinoma cells were separated from the bulk of other microsomal components in Ficoll density gradients. Initial separation depends on the differential action of magnesium ion on various microsomal components. Immunologic and enzymic markers were used to monitor separation and purification steps.Keywords
This publication has 9 references indexed in Scilit:
- The binding of kidney-bean phytohemagglutinin by Ehrlich ascites carcinomaBiochimica et Biophysica Acta (BBA) - General Subjects, 1965
- Studies on the surface and cytoplasmic membranes of Ehrlich ascites carcinoma cellsBiochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects, 1964
- PLASMA AND CYTOPLASMIC MEMBRANE FRAGMENTS FROM EHRLICH ASCITES CARCINOMAProceedings of the National Academy of Sciences, 1964
- Studies on plasma membranes I. Chemical composition and enzyme content of plasma membranes isolated from rat liverBiochimica et Biophysica Acta (BBA) - General Subjects, 1964
- Association of Cell Surface Antigens with Microsomal Membrane Fractions derived from Ehrlich Ascites Carcinoma CellsNature, 1962
- Studies on the surface and cytoplasmic membranes of Ehrlich ascites-carcinoma cellsBiochimica et Biophysica Acta, 1962
- Sialic acid in the cellular membranes of Ehrlich ascites-carcinoma cellsBiochimica et Biophysica Acta, 1961
- Pressure homogenization of mammalian tissuesBiochimica et Biophysica Acta, 1961
- THE ISOLATION OF A CELL MEMBRANE FRACTION FROM RAT LIVERThe Journal of cell biology, 1960