Estimation of serum haemoglobin-binding capacity (haptoglobin) on Sephadex G.100

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Abstract
A method is described for the estimation of serum haptoglobin as `haemoglobin-binding capacity'. The method relies on column chromatographic separation of the haemoglobin/haptoglobin complex from excess added free haemoglobin on the dextran gel Sephadex G.100. The method is simple and reproducible, and correlates well with another method of estimating haemoglobin-binding capacity over a wide range of values.