Abstract
Eggs from A. suum were cultured to the infective stage in 2% formalin. Infective eggs were treated at 37.5C in a soln. of 2% commercial Na hypochlorite (5.25%) prepn., "Clorox" and 2% NaOH and washed with physiol. saline. When these eggs were subsequently incubated in saline at 37.5 and 40[degree]C hatching occurred up to 100% in 4 weeks, but most larvae did not survive the hatching period. Batches of treated and washed eggs that were dried, rewet and incubated in saline at 37.5[degree]C hatched as high as 100% in 2 days. Up to 83.5% of the liberated larvae were alive in the hatching media 24 hrs. later. When treated eggs were centrifuged in saline for 10 min. at 3000 R.P.M. with a starting temp, of 40C, hatching up to 99.1% was produced. With this method the larvae were available immediately after centrifuging and up to 87% of the liberated larvae were motile 24 hrs. after hatching.
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