Disruption of Three Acid Proteases in Aspergillus Niger— Effects on Protease Spectrum, Intracellular Proteolysis, and Degradation of Target Proteins

Open Access
Publisher Website
Google Scholar
Abstract
Three acid protease genes encoding two extracellular proteases (PEPA and PEPB) and one intracellular protease (PEPE) were disrupted in Aspergillus niger. Northern‐blot analysis showed the absence of wild‐type protease mRNAs in the disruptants while western‐blot analysis proved the absence of the encoded proteases. Characterization of the residual proteolytic spectra in the disruptants indicated that the extracellular protease activity was reduced to 16% and 94% for the DpepA and the DpepB disruptants, repectively. In the DpepE disruptant, the total intracellular proteolytic activity was reduced to 32%. Apart from the reduced intracellular pepstatin‐inhibitable aspartyl protease activity, serine protease and serine carboxypeptidase activities were also significantly reduced in the DpepE strain. This may indicate the presence of a cascade activation mechanism for several vacuolar proteases, triggered by the PEPE protein, similar to the situation in Saccharomyces cerevisiae. Disruption of a single protease gene had no effects on the transcription of other non‐disrupted protease genes in A. niger. In supernatants of the disruptants, reduced degradation of a proteolytically very susceptible tester protein (PELB) was observed. By recombination, we also constructed DpepADpepB, DpepBDpepE and DpepADpepE double disruptants as well as a DpepADpepB DpepE triple disruptant, lacking all three acid protease activities. The in vitro residual PELB activity was the highest in the triple disruptant and the DpepADpepB recombinant.