Removal of RNase activity from DNase by affinity chromatography on agarose-coupled aminophenylphosphoryl-uridlne-2′(3′)-phosphate
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (1) , 241-246
- https://doi.org/10.1093/nar/4.1.241
Abstract
Severe degradation of high molecular weight RNA was shown to occur during incubation with commercially purified DNase. Most of the RNase activity could be removed by passage of the DNase through a column of agarose-coupled amino phenylphosphoryl-uridine-2' (3')-phosphate. Incubation with the treated DNase caused only minimal alteration of the sedimentation pattern of high molecular weight nuclear RNA, determined under partially denturing conditions. No impairment of DNase activity was detected.Keywords
This publication has 5 references indexed in Scilit:
- Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNAAnalytical Biochemistry, 1976
- Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteriaBiochemical Journal, 1967
- Isolation of deoxyribonucleic acid from mammalian tissuesBiochemical Journal, 1967
- The ribonuclease activity of crystallized pancreatic deoxyribonucleaseAnalytical Biochemistry, 1966