Über das Co-Ferment der Glykolyse aus Tumoren.

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Abstract
The particular enzymic part of the glycolytic process which caused the increased lactic acid formation found by 0. Warburg in tumors was studied. The glycolytic coferment, whose presence in tumors had been established by N. Waterman, and by H. v. Euler and K. Myrback, was therefore determined for human mammary, rectal and gastric carcinomas, Flexner-Jobling rat carcinomas and Jensen rat sarcomas. The tests were made on washed sections of rat kidney, the glycolysis of which was increased by tumor extracts almost up to the level of the tumors themselves. The quantity of lactic acid produced was determined by Warburg''s manometric method. Comparisons were made against extracts of kidney and liver tissues which also contained the coferment but in smaller quantities than did tumors. The coferment extracted from the tumors was concentrated to about 125 times by alcohol precipitation and adsorption on clay; it was tested by finding how the glycolysis of fresh liver and kidney sections was influenced by addition of this purified coferment. This study showed that the coferment was the limiting factor for glycolysis by normal kidney and liver tissue. Carcinomatous tissue differed from the latter by its increased content of coferment.

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