A New Stain Mixture: Aceto-Orcein-Fast-Green

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Abstract
A new mixture consisting of aceto-orcein, fast green, and salt is described. The mixture provides contrast between nuclear material, nucleoli and cytoplasm without differentiation. We have found it most useful in the staining of teased tissues, squashes, smears and suspensions (fixed and unfixed). We recommend it for the great speed and simplicity with which it provides a stain having the contrast of much more laborious histological technics.

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