Isolation of semliki forest virus from Aedes (Aedimorphus) argenteopunctatus (theobald) collected in Portuguese East Africa

Abstract
When the whole venom of Enydrina schistosa is injected into the medulla oblongata of rabbits death results even with small doses. If equivalent doses of a fraction containing the lecithinase activity are used, similar effects on the medulla are observed although this fraction is non-toxic when injected intraperitoneally. On the other hand, the neurotoxic fraction, which acts similarly to the whole venom when injected intraperitoneally, has virtually no effect on the medulla. Evidence is presented that the initial effect of the venom on the muscle is at the neruromuscular junction and that this may be temporary. With high doses a permanent muscle damage develops later.