Structural complexity of primary cultures of astrocytes as revealed by transverse sections
- 1 January 1988
- Vol. 1 (2) , 151-155
- https://doi.org/10.1002/glia.440010207
Abstract
The tissue architecture of low-, medium-, and high-density primary mouse astroglial cultures was examined in horizontal and transverse planes using the electron microscope. It was found that the low-density (colony) cultures consisted of a true monolayer, whereas the medium- and high-density (confluent) cultures consisted of anywhere from two to seven overlapping sheets enclosing a substantial intercellular space. The presence of these multiple overlapping sheets in confluent astrocyte cultures should therefore be taken into consideration when interpreting data of cell-membrane-related phenomena such as ion fluxes.Keywords
This publication has 14 references indexed in Scilit:
- Procedure for establishing oligodendroglial cells in primary cultures based on developmental parametersInternational Journal of Developmental Neuroscience, 1987
- Primary astroglial culturesNeurochemical Research, 1986
- High-affinity uptake of taurine and ?-alanine in primary cultures of rat astrocytesNeurochemical Research, 1986
- Binding of [3H]Ro 5–4864 in primary cultures of astrocytesBrain Research, 1985
- Plasticity of Astrocytes in Primary Cultures: An Experimental Tool and a Reason for Methodological CautionDevelopmental Neuroscience, 1985
- Fibrous and protoplasmic astrocytes are biochemically and developmentally distinctJournal of Neuroscience, 1984
- Astrocyte cell lineage. III. The morphology of differentiating mouse astrocytes in colony cultureJournal of Neurocytology, 1984
- Astrocyte cell lineage. I. Astrocyte progenitor cells in mouse neopalliumJournal of Comparative Neurology, 1981
- Fine structure of cultured glioblasts before and after stimulation by a Glia Maturation FactorExperimental Cell Research, 1977
- Nigrosin as a dye for differentiating live and dead ascites cellsExperimental Cell Research, 1958