Identification, Characterization, and Variable Expression of a Naturally Occurring Inhibitor Protein of IS 1106 Transposase in Clinical Isolates of Neisseria meningitidis
- 1 December 2001
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (12) , 7425-7436
- https://doi.org/10.1128/iai.69.12.7425-7436.2001
Abstract
Transposition plays a role in the epidemiology and pathogenesis of Neisseria meningitidis . Insertion sequences are involved in reversible capsulation and insertional inactivation of virulence genes encoding outer membrane proteins. In this study, we have investigated and identified one way in which transposon IS 1106 controls its own activity. We have characterized a naturally occurring protein (Tip) that inhibits the transposase. The inhibitor protein is a truncated version of the IS 1106 transposase lacking the NH 2 -terminal DNA binding sequence, and it regulates transposition by competing with the transposase for binding to the outside ends of IS 1106 , as shown by gel shift and in vitro transposition assays. IS 1106 Tip mRNA is variably expressed among serogroup B meningococcal clinical isolates, and it is absent in most collection strains belonging to hypervirulent lineages.This publication has 71 references indexed in Scilit:
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