Flagellar movement in demembranated preparations of ejaculated fowl spermatozoa

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Abstract
Triton X-100 at a concentration of 0.1% in the extraction medium was optimal for demembranating fowl spermatozoa. The most suitable conditions for reactivation were obtained when a medium composed of 0.5 mM-ATP, 25 mM-potassium glutamate, 10-7 M-CaCl2, 20 mM-Tris-HCl(pH 7.9), 1 mM-MgSO4, 1mM-dithiothreitol and 0.2 m-sucrose was used. More than 60% motile spermatozoa were obtained under these conditions. The addition of 1 or 10 .mu.M-cAMP did not appreciably affect motility. Intact and demembranated spermatozoa were immotile at 40.degree.C, whilst at 30.degree.C motility was restored.

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