Nucleolus and Chromosomes in Euglena gracilis

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Abstract
Nuclear constituents were degraded by nucleases and proteinases. Patterns of depolymerization are described in interphaslc and in mitotic stages of Euglena gracilis var. bacillaris treated in vivo with DNAse, RNAse, trypsin and [alpha]-chymotripsin for exposure extended from 2 to 6 hours. DNAse degraded chromatin at all stages, and it also hydrolyzed a nucleolar constituent. RNAse moderately degraded one nucleolar component and certain regions in the pro-phase chromosomes. Observations on control cells and RNAse-treated cells showed that the nucleolus possesses highly coiled filamentous structures which resemble chromonemata and extend into the nucleolar chromosomes. Treatment by nuclease followed by proteinase demonstrated that the nucleolar filamentous structure resembling chromonemata was degraded by DNAse and by either proteinase but was RNAse-resistant. This nucleolar structure is not regarded as the nucleolonema but as the nucleolar organizer proper, and as such chromosomal in nature.

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