Peptide Chain Termination with Mammalian Release Factor
- 1 September 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 67 (1) , 99-106
- https://doi.org/10.1073/pnas.67.1.99
Abstract
We report a method for the in vitro study of peptide chain termination in mammals. A proteinaceous release factor has been isolated from rabbit reticulocyte extracts. This factor and a polyribonucleotide template containing the bases U and A stimulate the release of N-formylmethionine from mammalian [N-formylmethionyl-tRNA(f).ribosome] intermediates. Our studies suggest that UAA is a terminator codon for mammalian cells.Keywords
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