The Mode of Action of Bacilysin and Anticapsin and Biochemical Properties of Bacilysin-resistant Mutants

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Abstract
Bacilysin is hydrolyzed to L-alanine and anticapsin by suspensions of a bacilysin-sensitive strain of Staphylococcus aureus but not by those of a resistant strain derived from it. It is hydrolyzed by extracts of both strains. Anticapsin is a powerful inhibitor of glucosamine synthetase in extracts of both the bacilysin-sensitive and resistant strains of S. aureus. Bacilysin, by comparison, is a relatively poor inhibitor of glucosamine synthetase in crude extracts when its hydrolysis in inhibited by EDTA. A phenylalanine auxotroph of S. aureus readily uses L-alanyl-L-phenylalanine for growth, but a bacilysin-resistant mutant depends on its transport into the organism, its hydrolysis to anticapsin and on inhibition by the latter of glucosamine synthetase; bacilysin-resistant mutants are apparently defective in a transport system.

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