Detachment of Segments from DNA Double Strands as Detected by Time Resolved Rayleigh Light Scattering
Open Access
- 1 August 1976
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 31 (7-8) , 484-485
- https://doi.org/10.1515/znc-1976-7-827
Abstract
Calf thymus DNA was irradiated in O2-saturated 0.01 N NaCl solution with 2 .mu.s pulses of 15 MeV electrons. By monitoring the decrease of light scattering intensity after the pulse, 2 modes of decrease were detected. The fast decrease (.tau.1/2 .apprxeq. 0.8 ms) is ascribed to the separation of DNA fragments produced by double strand breaks at positions directly opposite to each other. The slow decrease (.tau.1/2 .apprxeq. 8 s) is attributed to the detachment of segments generated by single strand breaks at sites on the alternate strands being separated by about 10 nucleotide units.Keywords
This publication has 1 reference indexed in Scilit:
- Bestimmung von Einzel- und Doppelbrüchen in bestrahlter desoxyribonukleinsäure durch die MolekulargewichtsverteilungBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967