Multiple selective media for the isolation of anaerobic bacteria from clinical specimens.
Open Access
- 1 January 1980
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 33 (1) , 61-65
- https://doi.org/10.1136/jcp.33.1.61
Abstract
Using fresh clinical material, a comparison of a number of anaerobic selective media was made. For Gram-negative anaerobes nalidixic acid tween agar (NAT), neomycin agar (NA), and neomycin-vancomycin agar (NVA) all performed equally well. Kanamycin-containing media were more inhibitory to all Gram-negative anaerobes other than Bacteroides fragilis and B. melaninogenicus. When the recovery of Gram-positive anaerobes was examined NAT performed better than any of the other selective media used. No single selective medium could recover all anaerobes. Better isolation was achieved using a combination of two selective media (the best combinations being NAT and NVA or NAT and NA). Only a combination of three selective media gave the maximum recovery of anaerobes in this study (NAT, NVA, and NA or KA).This publication has 9 references indexed in Scilit:
- A new selective medium for the isolation of non-sporing anaerobic bacteria from clinical specimens.1978
- The Anaerobic Culture of Clinical Specimens: A 14-Month StudyJournal of Medical Microbiology, 1977
- Tests of performance of anaerobic jars.Journal of Clinical Pathology, 1976
- Anaerobes survive in clinical specimens despite delayed processingJournal of Clinical Microbiology, 1976
- An improved catalyst sachet for anaerobic jars.1975
- Isolation and indentification of anaerobic bacteria in the clinical laboratory. A 2-year experience.1974
- Comparison of Three Anaerobic Systems for the Isolation of Anaerobic Bacteria from Clinical SpecimensAmerican Journal of Clinical Pathology, 1973