13C-NMR Spectroscopy of Human Serum High Density Lipoprotein Enriched with Labelled Phospholipids
- 1 January 1979
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 360 (1) , 523-528
- https://doi.org/10.1515/bchm2.1979.360.1.523
Abstract
Native human serum high density lipoprotein (HDL) (density = 1.063-1.21 g .times. cm-3) was enriched with phosphatidylcholines labeled with 13C in the polar head group ([N-13CH3]choline) and in the fatty acyl chains ([26-13C]cholesterol) and its linoleic acid ester using the previously described exchange method (Stoffel et al.). The properties of the HDL particles with the exchanged lipid classes were the same as those of the native particles (MW, circular dichroism, fluorescence, lipid and apoprotein stoichiometry, electrophoretic mobility). The T1 times were very similar to those obtained previously with recombined apolipoprotein-[13C]lipid complexes.This publication has 4 references indexed in Scilit:
- A New Method for the Exchange of Lipid Classes of Human Serum High Density LipoproteinHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1978
- Lipid-Protein Interactions between Human Apolipoprotein A-I and Defined Sphingomyelin Species. A13C-NMR Spectroscopic StudyHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1977
- Human High Density Apolipoprotein A-I-Lysolecithin-Lecithin and Sphingomyelin Complexes. A Method for High Yield Recombinations to Lipoprotein Complexes of Reproducible StoichiometryHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1976
- 13C-Nuclear Magnetic Resonance Spectroscopic Studies on Saturated, Mono-, Di- and Polyunsaturated Fatty Acids, Phospho- and Sphingo- lipidsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1972