Identification of an essential sequence for dihydroceramide C‐4 hydroxylase activity of mouse DES2
- 11 September 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 576 (1-2) , 63-67
- https://doi.org/10.1016/j.febslet.2004.08.060
Abstract
Although the amino acid sequences of mouse DES1 (MDES1) and DES2 (MDES2) have 63% sequence identity, their enzymatic characteristics are quite different. MDES1 exhibits high dihydroceramide Δ4-desaturase activity and very low C-4 hydroxylase activity, while MDES2 is similarly active as both a dihydroceramide Δ4-desaturase and a C-4 hydroxylase. We constructed several chimeras of MDES1 and MDES2 and identified a region important for C-4 hydroxylase activity in MDES2. This region contains the sequence XAFGY (X=T or A or V; Y=T or N) and occurs on the C-terminal side of the first His-box of MDES2. We confirmed the conservation of this region in DES2 family members sequenced from humans, pigs, rats, chickens, zebrafish, and XenopusKeywords
This publication has 20 references indexed in Scilit:
- DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestineBiochemical Journal, 2004
- Structure–function studies of yeast C-4 sphingolipid long chain base hydroxylaseBiochimica et Biophysica Acta (BBA) - Biomembranes, 2003
- Stereochemistry of a bifunctional dihydroceramide Δ4-desaturase/hydroxylase from Candida albicans; a key enzyme of sphingolipid metabolismOrganic & Biomolecular Chemistry, 2003
- De Novo Sphingolipid Biosynthesis: A Necessary, but Dangerous, PathwayJournal of Biological Chemistry, 2002
- A Small Region of the Natural Killer Cell Receptor, Siglec-7, Is Responsible for Its Preferred Binding to α2,8-Disialyl and Branched α2,6-Sialyl ResiduesJournal of Biological Chemistry, 2002
- Role of Highly Conserved Residues in the Reaction Catalyzed by Recombinant Δ7-Sterol-C5(6)-Desaturase Studied by Site-Directed MutagenesisBiochemistry, 2000
- degenerative spermatocyte, a novel gene encoding a transmembrane protein required for the initiation of meiosis in Drosophila spermatogenesisMolecular Genetics and Genomics, 1996
- Site‐directed mutagenesis of histidine residues in the Δ12 acyl‐lipid desaturase of SynechocystisFEBS Letters, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Eight Histidine Residues Are Catalytically Essential in a Membrane-Associated Iron Enzyme, Stearoyl-CoA Desaturase, and Are Conserved in Alkane Hydroxylase and Xylene MonooxygenaseBiochemistry, 1994