New approaches to the role of sulfhydryl groups in silver stainability of protein in grasshopper chromosomes

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Abstract
Silver staining in somatic and germ line cells of the grasshopper Arcyptera fusca has been analyzed after a standard silver staining technique was used with pretreatments that included dithiothreitol and β-mercaptoethanol as reagents to maintain —SH groups in the reduced state. The results show that in some tissues, these pretreatments improve not only the silver staining of nucleolar masses but also the recognition of other silver spots associated with the chromatin. These observations are similar to those previously described for the effect of double-strength standard saline citrate on silver staining. The possible chemical nature of the protein groups responsible for the differential silver stainability and the role of saline citrate in the modification of argyrophylic proteins suggested previously are briefly discussed.Key words: Orthoptera, silver staining, sulfhydryl groups.

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