NMR study of parallel-stranded tetraplex formation by the hexadeoxynucleotide d(TG4T)
- 1 November 1992
- journal article
- Published by Springer Nature in Nature
- Vol. 360 (6401) , 280-282
- https://doi.org/10.1038/360280a0
Abstract
Multistranded DNA structures based upon guanine association have been proposed to be important in the structure of chromosome telomeres and in immunoglobulin class switching. Nucleic acids containing runs of guanine bases form a number of structures in vitro, including fold-back structures (Fig. 1a) and parallel-stranded quadruplex structures in DNA and RNA. The features of fold-back structures have now been determined at high-resolution. The different structures are probably based on a tetrad of hydrogen-bonded guanine bases (Fig. 1b), with buffer conditions and sequence effects mediating isomerization between the different forms. Here we use NMR spectroscopy to investigate the solution structure of the complex formed by the hexadeoxynucleotide d(TG4T) in the presence of sodium ions. We have observed the formation of a parallel-stranded quadruplex containing hydrogen-bonded tetrads of guanine. The parallel-stranded form differs significantly from the fold-back form, with individual nucleotide conformations being closer to those of B-form DNA.Keywords
This publication has 29 references indexed in Scilit:
- Quadruplex structure of Oxytricha telomeric DNA oligonucleotidesNature, 1992
- Cation-dependent transition between the quadruplex and Watson-Crick hairpin forms of d(CGCG3GCG)Biochemistry, 1992
- Multinuclear nuclear magnetic resonance studies of Na cation-stabilized complex formed by d(G-G-T-T-T-T-C-G-G) in solution: Implications for G-tetrad structuresJournal of Molecular Biology, 1991
- Tetramerization of an RNA oligonucleotide containing a GGGG sequenceNature, 1991
- Structure and function of telomeresNature, 1991
- A sodium-potassium switch in the formation of four-stranded G4-DNANature, 1990
- NOEMOL: integrated molecular graphics and the simulation of Nuclear Overhauser effects in NMK spectroscopyJournal of Molecular Graphics, 1989
- Formation of parallel four-stranded complexes by guanine-rich motifs in DNA and its implications for meiosisNature, 1988
- Assignment of the 31P and 1H resonances in oligonucleotides by two‐dimensional NMR spectroscopyFEBS Letters, 1986
- Assignment of the non-exchangeable proton resonances of d(C-G-C-G-A-A-T-T-C-G-C-G) using two-dimensional nuclear magnetic resonance methodsJournal of Molecular Biology, 1983