Free Fatty Acids Identified as Antitryptic Factor in Soybeans Fermented by Rhizopus oligosporus

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Abstract
The trypsin-inhibitory activity observed in cooked soybeans fermented by Rhizopus oligosporus (fungus used in tempeh fermentation) has been examined. The active compounds have now been isolated by ethanol extraction and thin-layer chromatography and have been identified as free fatty acids by infrared spectroscopy and gasliquid chromatography. Oleic, linoleic, and linolenic acids are primarily responsible for the increased trypsin-inhibiting activity of cooked soybeans after fermentaton. The free fatty acids are liberated from oil in the soybeans by fungal lipase, and they differ from other reported soybean trypsin inhibitors that are protein in nature. Free fatty acids have been previously reported to inhibit various enzymes, such as glycolytic, glyconeogenic, lipogenic, and also proteolytic. Their effect appears to be a nonspecific type of inhibition. Further studies are required to determine their physiological relevance, if any.