The Regulation by Growth Hormone of Microsomal Testosterone 6β-Hydroxylase in Male Rat Livers1
- 1 October 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 100 (4) , 1095-1097
- https://doi.org/10.1093/oxfordjournals.jbchem.a121790
Abstract
The relationship between growth hormone and testosterone (T) 6β-hydroxylase was investigated in normal and hypophysectomized (hypox) male rats. The administration of human growth hormone (hGH) by either intermittent injections or continuous infusion to normal and hypox rats decreased the activity of T 6β-hydroxylase in hepatic microsomes. Hypophysectomy did not reduce, but rather increased the 6β-hydroxylase activity, while the 16α-hydroxylase activity decreased. These results, together with the data from a Western blot, indicate that growth hormone acts as a repressive factor for the expression of T 6β-hydroxylase in a manner different from the regulation of T 16α-hydroxylase.This publication has 11 references indexed in Scilit:
- Regulation of rat hepatic cytochrome P-450: age-dependent expression, hormonal imprinting, and xenobiotic inducibility of sex-specific isoenzymesBiochemistry, 1985
- Activation and inactivation of a variety of mutagenic compounds by the reconstituted system containing highly purified preparations of cytochrome P-450 from rat liverFundamental and Applied Toxicology, 1985
- AGE-RELATED ALTERATION IN THE ACTIVITIES OF DRUG-METABOLIZING-ENZYMES AND CONTENTS OF SEX-SPECIFIC FORMS OF CYTOCHROME-P-450 IN LIVER-MICROSOMES FROM MALE AND FEMALE RATS1985
- Characterization of three highly purified cytochromes P-450 from hepatic microsomes of adult male rats.Journal of Biological Chemistry, 1984
- Testosterone metabolism by cytochrome P-450 isozymes RLM3 and RLM5 and by microsomes. Metabolite identification.Journal of Biological Chemistry, 1983
- Sex difference of cytochrome P-450 in the rat: Purification, characterization, and quantitation of constitutive forms of cytochrome P-450 from liver microsomes of male and female ratsArchives of Biochemistry and Biophysics, 1983
- Hepatic microsomal cytochrome P-450 from rats treated with isosafrole. Purification and characterization of four enzymic forms.Journal of Biological Chemistry, 1980
- Separation, purification, and characterization of a novel form of hepatic cytochrome P-450 from rats treated with pregnenolone-16 alpha-carbonitrile.Journal of Biological Chemistry, 1980
- Age- and Sex-Related Differences in Episodic Growth Hormone Secretion in the Rat*Endocrinology, 1979
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951