Measurement of aqueous humor flow with scanning ocular fluorophotometers

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Abstract
A 1-dimensional scanning fluorophotometer (Fluorotron Master) and a 2-dimensional scanning fluorophotometer (McLaren and Brubaker) were used to measure rate of aqueous humor flow in normal volunteers. The precision of 3 protocols of dye application and flow determination was investigated. In all 3 protocols rate of flow was determined by observing the rate of disappearance of topically-applied fluorescein during an 8-h period. The 3 protocols studied were the following: 2 drops of 10% fluorescein washed away 5 min later immediately before beginning measurements of fluorescence. Application of 0.25% fluorescein every 5 min for 30 min, 6 h before beginning measurements iontophoresis for 7 s and irrigation of eye immediately before beginning measurements. Rate of flow was calculated by the methods of Jones and Maurice. The results were compared to measurements made with a slit-lamp fluorophotometer using the technique described by Coakes and Brubaker. The most satisfactory method of measuring flow when either scanning fluorophotometer was employed was to instill fluorescein many hours before measurements begin (the 2nd protocol).

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