A SCREENING TEST OF THE EFFECT OF ORGANIC COMPOUNDS ON PRODUCTION OF TOBACCO MOSAIC VIRUS

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Abstract
A number of compounds were tested for their effect on production of TMV in floating leaf discs. Two environments were used: diffuse daylight (25 footcandles) and laboratory temp., and artificial light (300 f .c.) for 12 hours per day at 4 to 5 C above laboratory temp. Approx. twice as much virus was produced in artificial light environment as in diffuse daylight environment. A wide range of organic compounds increased virus production in diffuse daylight environment but had less effect, or inhibited virus production, in artificial light environment. It was suggested that these compounds may furnish energy for metabolism of tobacco tissues and in this way cause an increase in virus production, particularly in diffuse daylight environment where host metabolites are depleted. The following compounds were among most effective in increasing virus production: glucose-1-phosphate, 6-methyluracil, propyl thiouracil, isocytosine, and glucose. The following compounds were most effective in inhibiting virus production: thiouracil, diazouracil, zinc chloride and L-isoleucine.
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