A Rapid Preparative Procedure for the Treatment of Free Urinary Catecholamines

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Abstract
We report a preparative and liquid chromatographic method for the determination of free urinary catecholamines in urine. An extraction column device (1) was used to isolate norepinephrine, epinephrine on a weak cation exchange resin (Bio-Rex 70) using a table centrifuge forcing the sample through the resin material. The preparation time for a 8 sample size was 50 minutes. The extraction recovery was 80.33% and 80.89% for norepinephrine (NE) and epinephrine (E). The curve was linear up to 400 ug/24 H. The within-run CV was 3.84% and 4.22% for NE and E; the between-run CV was 5.58% and 6.27% respectively for NE and E. The mobile phase consisted of a 90% phosphate buffer (0.05M NA2HP04) and 10% methanol adjusted to pH. 3.8. Chromatographically well separated on a C-8 reverse phase column, the eluent was monitored with a coulometric electrochemical detector set up at 0.40 V.