The Behaviour of Nucleic Acids and Other Constituents inProtomyces inundatusDangeard

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Abstract
Estimations of contents per cell of DNA and RNA were made in Protomyces inundatus. There was twice as much DNA per cell in cultures derived from fusion bodies and from mycelium in the host (Apium nodiflorum) as in those derived from unfused endospores, indicating that the former two were diploid, the latter haploid. Dry weight/cell, volume/cell, insoluble nitrogen/cell, and metaphosphate/cell, like RNA/cell, had similar values for all three types of cultures. Giant cells resulting from camphor treatment not only had twice as much DNA/cell as normal cultures derived from unfused endospores, but all the other characteristics studied had doubled as well. In any one culture the ratio of RNA to DNA remained constant though the absolute values decreased as the cultures aged. The RNA to DNA ratios in P. inundatus of haploid and diploid cultures are compared to those found in yeast and other organisms.

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